WB (peptide competition) analysis of PMA-treated K652 cell lysates using GTX25785 PKC beta 2 (phospho Thr641) antibody with the non-phosphopeptide (Lane 2), a generic (pT) peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.