PKC epsilon (phospho Ser729) antibody detects PKC epsilon (phospho Ser729) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PKC epsilon (phospho Ser729) stained by PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PKC epsilon (phospho Ser729) antibody detects PKC epsilon (phospho Ser729) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PKC epsilon (phospho Ser729) stained by PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC epsilon (phospho Ser729) antibody (GTX133937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.