GeneTex
United States (US)

PKC nu (Active) recombinant protein

Cat No. GTX65399

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

177 nmol phosphate incorporated into CREBTIDE substrate peptide per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

142.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥80%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PRKD3, PKC, Protein kinase C nu

Background

PKCnu, also known as PKD3, is a members of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCnu is composed of 890 amino acid residues and the protein has 77.3% similarity to human PKC mu (PKCmu) and 77. 4% similarity to 22 PKD (the 22 homolog of PKCmu) (1). The PKCnu mRNA is ubiquitously expressed in various tissues and the gene is located between markers WI-9798 and D2S177 on chromosome 2p21 region.PKCnu has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCnu is trans-located to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCnu is an important physiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induced a rapid activation of PKCnu by a protein kinase C (PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCnu. PKCnu is present both in the nucleus and cytoplasm and this distribution of PKCnu results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induced a rapid and reversible plasma membrane translocation of PKCnu that is PKC-dependent.
Package List Price ($)
$ 349