Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKR antibody (GTX132826) diluted at 1:1000.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKR antibody (GTX132826) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.