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PLCB1 antibody

Anti-PLCB1 antibody used in Western Blot (WB). GTX54594
Anti-PLCB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54594
Anti-PLCB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54594
Anti-PLCB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54594
Anti-PLCB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54594

Cat. No. GTX54594

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all PLCB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase C beta 1 , EIEE12 , PI-PLC , PLC-154 , PLC-I , PLC-beta-1 , PLC154 , PLCB1A , PLCB1B

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PLCB1 antibody used in Western Blot (WB). GTX54594

GTX54594 WB Image

WB analysis of NCI-H460 cell lysate using GTX54594 PLCB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PLCB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54594

GTX54594 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54594 PLCB1 antibody.
Blue : DAPI

Anti-PLCB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54594

GTX54594 IHC-P Image

IHC-P analysis of rat brain tissue using GTX54594 PLCB1 antibody.
Dilution : 1:100

Anti-PLCB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54594

GTX54594 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX54594 PLCB1 antibody.
Dilution : 1:100

Anti-PLCB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54594

GTX54594 IHC-P Image

IHC-P analysis of rat heart tissue using GTX54594 PLCB1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399