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PLCB2 antibody

Comparison Orthogonal
Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765
Anti-PLCB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133765
Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765
Anti-PLCB2 antibody used in Western Blot (WB). GTX133765
Anti-PLCB2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133765
Anti-PLCB2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133765

Cat. No. GTX133765

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PLCB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PLCB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887097

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase C beta 2 , PLC-beta-2

Database

Research Area

DATA IMAGES

Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765

GTX133765 IHC-P Image

PLCB2 antibody detects PLCB2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PLCB2 stained by PLCB2 antibody (GTX133765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PLCB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133765

GTX133765 ICC/IF Image

PLCB2 antibody detects PLCB2 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLCB2 stained by PLCB2 antibody (GTX133765) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765

GTX133765 IHC-P Image

PLCB2 antibody detects PLCB2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
PLCB2 stained by PLCB2 antibody (GTX133765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PLCB2 antibody used in Western Blot (WB). GTX133765

GTX133765 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PLCB2 antibody (GTX133765) diluted at 1:1000 and competitor's antibody (sc-206) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PLCB2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133765

GTX133765 IHC-Fr Image

Orthogonal

The data was published in the journal Life Sci Alliance in 2019. PMID: 31167803

Anti-PLCB2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133765

GTX133765 IHC-Fr Image

The data was published in the journal Life Sci Alliance in 2019. PMID: 31167803

REFERENCE

Application Reference

Gao Y et al. Life Sci Alliance 2019; 2 (3) The WT1-BASP1 complex is required to maintain the differentiated state of taste receptor cells.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169