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PLCB2 antibody

Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765
Anti-PLCB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133765
Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765
Anti-PLCB2 antibody used in Western Blot (WB). GTX133765

Cat No. GTX133765

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Human PLCB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887097

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phospholipase C beta 2 , PLC-beta-2

Database

Research Area

DATA IMAGES

Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765

GTX133765 IHC-P Image

PLCB2 antibody detects PLCB2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PLCB2 stained by PLCB2 antibody (GTX133765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PLCB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133765

GTX133765 ICC/IF Image

PLCB2 antibody detects PLCB2 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLCB2 stained by PLCB2 antibody (GTX133765) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PLCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133765

GTX133765 IHC-P Image

PLCB2 antibody detects PLCB2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
PLCB2 stained by PLCB2 antibody (GTX133765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PLCB2 antibody used in Western Blot (WB). GTX133765

GTX133765 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PLCB2 antibody (GTX133765) diluted at 1:1000 and competitor's antibody (sc-206) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

REVIEW

PLCB2 antibody Cat No. GTX133765

Rating ( Average 5 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Life Sci Alliance in 2019.PMID: 31167803
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Life Sci Alliance in 2019.PMID: 31167803
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf