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PLCE1 antibody

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in Western Blot (WB). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580
Anti-PLCE1 antibody used in Western Blot (WB). GTX02580
Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

Cat No. GTX02580

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

259 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PLCE1, corresponding to a region within C-terminal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phospholipase C epsilon 1 , NPHS3 , PLCE , PPLC

Cellular Localization

Cytoplasm, cytosol , Cell membrane , Golgi apparatus membrane

Background

This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of mouse colon tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in Western Blot (WB). GTX02580

GTX02580 WB Image

WB analysis of HEK293 cell lysate using GTX02580 PLCE1 Antibody.

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02580

GTX02580 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02580 PLCE1 antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of rat thymus tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human normal tissues adjacent to pancreatic cancer using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human pancreatic cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of rat colon tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human normal tissues adjacent to colorectal cancer using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-PLCE1 antibody used in Western Blot (WB). GTX02580

GTX02580 WB Image

WB analysis of VERO cell lysate using GTX02580 PLCE1 Antibody. The lane on the left was treated with blocking peptide.

Anti-PLCE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02580

GTX02580 IHC-P Image

IHC-P analysis of human pancreatic cancer tissue using GTX02580 PLCE1 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

REFERENCE

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REVIEW

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