Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX122218 diluted at 1:1000

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PLD3 antibody [N1C1] (GTX122218) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.