Wild-type (WT) and PODXL knockout (KO) HeLa cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PODXL antibody [HL2047] (GTX637951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

PODXL antibody [HL2047] detects PODXL protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
PODXL stained by PODXL antibody [HL2047] (GTX637951) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PODXL antibody [HL2047] detects PODXL protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
PODXL stained by PODXL antibody [HL2047] (GTX637951) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PODXL antibody [HL2047] (GTX637951) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PODXL antibody [HL2047] detects PODXL protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PODXL stained by PODXL antibody [HL2047] (GTX637951) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min