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POFUT1 antibody [N1C1]

Anti-POFUT1 antibody [N1C1] used in Western Blot (WB). GTX119122
Anti-POFUT1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119122
Anti-POFUT1 antibody [N1C1] used in Western Blot (WB). GTX119122
Anti-POFUT1 antibody [N1C1] used in Western Blot (WB). GTX119122

Cat. No. GTX119122

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all POFUT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HUVEC , Mouse liver , HCT116

Predict Reactivity

Rat, Bovine, Dog, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human POFUT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617719

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein O-fucosyltransferase 1 , DDD2 , FUT12 , O-FUT , O-Fuc-T , O-FucT-1 , OFUCT1

Cellular Localization

Endoplasmic reticulum

Background

This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-POFUT1 antibody [N1C1] used in Western Blot (WB). GTX119122

GTX119122 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX119122 diluted at 1:1000

Anti-POFUT1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119122

GTX119122 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using POFUT1(GTX119122) antibody at 1:500 dilution.

Anti-POFUT1 antibody [N1C1] used in Western Blot (WB). GTX119122

GTX119122 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX119122 diluted at 1:1000

Anti-POFUT1 antibody [N1C1] used in Western Blot (WB). GTX119122

GTX119122 WB Image

Sample (15 ug of whole cell lysate)
A: HUVEC
12% SDS PAGE
GTX119122 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169