APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , 293T , A431 , PPP2CB-transfected 293T
Predict Reactivity
Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 40% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PP2A beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241202
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein phosphatase 2 catalytic subunit beta , PP2Abeta , PP2CB
Cellular Localization
Cytoplasm , Nucleus , Centromere , Spindle
Background
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX101690 WB Image
Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
12% SDS PAGE
GTX101690 diluted at 1:500
|
|
|
GTX101690 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP2A beta(GTX101690) antibody at 1:500 dilution.
|
|
|
GTX101690 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A beta antibody (GTX101690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101690 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101690 diluted at 1:1000
|
REFERENCE
There are currently no references for PP2A beta antibody (GTX101690). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PP2A beta antibody (GTX101690). Be the first to share your experience with this product.
