PPAR alpha antibody

Cat No. GTX101098

Reference 12

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP, ChIP assay
Reactivity	Human, Mouse, Rat, Honeybee, Queen Bee
Package	100 μl ($329), 25 μl ($169)

See all PPAR alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Rat primary hepatocyte , mouse liver , mouse brain , mouse lung

Predict Reactivity

Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR

Background

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq]

Database

Gene ID: 5465 PPARA
UniProt: Q07869 PPARA

Research Area

DATA IMAGES

GTX101098 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101098 WB Image

Rat tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101098 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
ChIP experiment and primer designs are based on J Biol Chem. 2013 Jan 18;288(3):1726-38.

GTX101098 IP Image

PPAR alpha antibody immunoprecipitates PPAR alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 μg of PPAR alpha antibody (GTX101098) 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody (GTX101098) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX101098 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.