GeneTex
United States (US)

PPAR alpha antibody

GTX101098_39764_20171117_WB_M_liver_19011506_996.jpg
GTX101098_39764_20171117_WB_M_tissue_19011506_726.jpg
GTX101098_39764_ChIP_19011506_128.jpg
GTX101098_39764_WB_Rat_19011506_965.jpg
GTX101098_39764_IP_19011506_818.jpg

Cat No. GTX101098

Reference 11
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ChIP assay
Reactivity Human, Mouse, Rat, Honeybee, Queen Bee
See all PPAR alpha products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Rat primary hepatocyte , mouse liver , mouse brain , mouse lung

Predict Reactivity

Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC2237 antibody, MGC2452 antibody, NR1C1 antibody, PPAR antibody, PPARalpha antibody, hPPAR antibody, PPARA antibody, peroxisome proliferator-activated receptor alpha antibody, PPAR-alpha antibody, peroxisome proliferator-activated nuclear receptor alpha variant 3 antibody, nuclear receptor subfamily 1 group C member 1 antibody, "peroxisome proliferative activated receptor, alpha antibody"

Background

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101098_39764_20171117_WB_M_liver_19011506_996.jpg

GTX101098 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101098_39764_20171117_WB_M_tissue_19011506_726.jpg

GTX101098 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101098_39764_ChIP_19011506_128.jpg

GTX101098 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
ChIP experiment and primer designs are based on J Biol Chem. 2013 Jan 18;288(3):1726-38.

GTX101098_39764_WB_Rat_19011506_965.jpg

GTX101098 WB Image

Sample (30 µg of whole cell lysate)
A: Rat primary hepatocyte
7.5% SDS PAGE
GTX101098 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101098_39764_IP_19011506_818.jpg

GTX101098 IP Image

PPAR alpha antibody immunoprecipitates PPAR alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 μg of PPAR alpha antibody (GTX101098) 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody (GTX101098) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
REFERENCE

Application Reference

Wang H et al. Am J Physiol Endocrinol Metab 2019; 316 (1):E54-E62 Adipose tissue transplantation ameliorates lipodystrophy-associated metabolic disorders in seipin-deficient mice.
Application : WB
Reactivity : Mouse
Orkhon B et al. Mol Med Rep 2018; (Epub ahead of print) Astragalus root induces ovarian β-oxidation and suppresses estrogen-dependent uterine proliferation.
Kubo H et al. Lipids Health Dis 2017; 16 (1):106 Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice.
Song L et al. Sci Rep 2017; 7:41604 Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet.
Chung IH et al. Oncotarget 2016; 7 (16):22448-59 ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance.
Application : WB
Reactivity : Human
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Submit a Reference
Application Reference
Wang H et al. Am J Physiol Endocrinol Metab 2019; 316 (1):E54-E62 Adipose tissue transplantation ameliorates lipodystrophy-associated metabolic disorders in seipin-deficient mice.
Application : WB
Reactivity : Mouse
Orkhon B et al. Mol Med Rep 2018; (Epub ahead of print) Astragalus root induces ovarian β-oxidation and suppresses estrogen-dependent uterine proliferation.
Kubo H et al. Lipids Health Dis 2017; 16 (1):106 Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice.
Song L et al. Sci Rep 2017; 7:41604 Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet.
Chung IH et al. Oncotarget 2016; 7 (16):22448-59 ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance.
Application : WB
Reactivity : Human
Ye JH et al. Sci Rep 2016; 6:33102 Pentoxifylline ameliorates non-alcoholic fatty liver disease in hyperglycaemic and dyslipidaemic mice by upregulating fatty acid β-oxidation.
Application : WB
Reactivity : Mouse
Xu P et al. Nutr Metab (Lond) 2015; 12:58 Diet rich in Docosahexaenoic Acid/Eicosapentaenoic Acid robustly ameliorates hepatic steatosis and insulin resistance in seipin deficient lipodystrophy mice.
Application : WB
Reactivity : Mouse
Hsu CY et al. Biogerontology 2014; Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees.
Application : WB
Reactivity : Queen Bee
Lai YS et al. J Agric Food Chem 2014; Garlic essential oil protects against obesity-triggered nonalcoholic fatty liver disease through modulation of lipid metabolism and oxidative stress.
Application : WB
Reactivity : Mouse
Hsu CY et al. J Gerontol A Biol Sci Med Sci 2013; Changes in Energy-Regulated Molecules in the Trophocytes and Fat Cells of Young and Old Worker Honeybees (Apis mellifera).
Application : WB
Reactivity : Honeybee
Ho FM et al. J Ethnopharmacol 2012; 142 (1):175-87 Anti-atherosclerotic action of Ger-Gen-Chyn-Lian-Tang and AMPK-dependent lipid lowering effect in hepatocytes.
Application : WB
Reactivity : Rat
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169