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PPAR alpha antibody

KO/KD Orthogonal Overexpression
Anti-PPAR alpha antibody used in Immunoprecipitation (IP). GTX101098
Anti-PPAR alpha antibody used in ChIP assay (ChIP assay). GTX101098
Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098
Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098
Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

Cat. No. GTX101098

Reference 20
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Honeybee, Queen Bee, Plant
Package
100 μl ($399),
25 μl ($169)
See all PPAR alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037654

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR

Background

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPAR alpha antibody used in Immunoprecipitation (IP). GTX101098

GTX101098 IP Image

PPAR alpha antibody immunoprecipitates PPAR alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 μg of PPAR alpha antibody (GTX101098) 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody (GTX101098) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-PPAR alpha antibody used in ChIP assay (ChIP assay). GTX101098

GTX101098 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
ChIP experiment and primer designs are based on J Biol Chem. 2013 Jan 18;288(3):1726-38.

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098

GTX101098 IHC-P Image

PPAR alpha antibody detects PPAR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PPAR alpha stained by PPAR alpha antibody (GTX101098) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098

GTX101098 IHC-P Image

PPAR alpha antibody detects PPAR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PPAR alpha stained by PPAR alpha antibody (GTX101098) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098

GTX101098 IHC-P Image

PPAR alpha antibody detects PPAR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PPAR alpha stained by PPAR alpha antibody (GTX101098) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

GTX101098 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

GTX101098 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Takuro Toda et al. Commun Biol 2022; 5 (1) : 453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.
Application : WB
Lin CH et al. Nutrients 2021; 13 (11) Consumption of Non-Nutritive Sweetener, Acesulfame Potassium Exacerbates Atherosclerosis through Dysregulation of Lipid Metabolism in ApoE-/- Mice.
Application : WB
Reactivity : Mouse
Chen HJ et al. Evid Based Complement Alternat Med 2021; 2021 : 9967910 Alleviative Effect of Ruellia tuberosa L. on Insulin Resistance and Abnormal Lipid Accumulation in TNF-α-Treated FL83B Mouse Hepatocytes.
Application : WB
Reactivity : Plant
Igarashi Y et al. Biomed Pharmacother 2021; 140 : 111714 Glavonoid-rich oil supplementation reduces stearoyl-coenzyme A desaturase 1 expression and improves systemic metabolism in diabetic, obese KK-Ay mice.
Application : WB
Dai J et al. Elife 2021; 10 Curcumin promotes AApoAII amyloidosis and peroxisome proliferation in mice by activating the PPARα signaling pathway.
Application : WB, IHC-P
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Submit a Reference

REVIEW

PPAR alpha antibody Cat. No. GTX101098

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the 2017 in Sci Rep. PMID: 28169366
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Date : Anonymous submitted on 23-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 50 μg
Blocking : Odyssey blocking buffer, 20°C, 1Hr
Primary Antibody : 1:500, 20°C, 1Hr
Figure Note : The band intensity of PPAR alpha was increased by transfection of PPAR alpha plasmid, and was decreased by transfection of siRNA for PPAR alpha.
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample : Brown adipocytes extract
Amount used : 50 μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Figure Note : CL: CL316243, adrenergic-beta 3 receptor agonist
Submit a Review
Application Reference
Takuro Toda et al. Commun Biol 2022; 5 (1):453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.
Application : WB
Lin CH et al. Nutrients 2021; 13 (11) Consumption of Non-Nutritive Sweetener, Acesulfame Potassium Exacerbates Atherosclerosis through Dysregulation of Lipid Metabolism in ApoE-/- Mice.
Application : WB
Reactivity : Mouse
Chen HJ et al. Evid Based Complement Alternat Med 2021; 2021:9967910 Alleviative Effect of Ruellia tuberosa L. on Insulin Resistance and Abnormal Lipid Accumulation in TNF-α-Treated FL83B Mouse Hepatocytes.
Application : WB
Reactivity : Plant
Igarashi Y et al. Biomed Pharmacother 2021; 140:111714 Glavonoid-rich oil supplementation reduces stearoyl-coenzyme A desaturase 1 expression and improves systemic metabolism in diabetic, obese KK-Ay mice.
Application : WB
Dai J et al. Elife 2021; 10 Curcumin promotes AApoAII amyloidosis and peroxisome proliferation in mice by activating the PPARα signaling pathway.
Application : WB, IHC-P
Wang CY et al. Int J Med Sci 2021; 18 (1):256-269 Upregulation of peroxisome proliferator-activated receptor-α and the lipid metabolism pathway promotes carcinogenesis of ampullary cancer.
Application : IHC-P
Reactivity : Human
Banerjee A et al. Sci Rep 2020; 10 (1):15443 Mechanistic study of attenuation of monosodium glutamate mixed high lipid diet induced systemic damage in rats by Coccinia grandis.
Application : WB
Ling T et al. J Med Chem 2019; 62 (15):6925-6940 Mechanistic Insight on the Mode of Action of Colletoic Acid.
Guo S et al. Brain Res 2019; 1722:146353 Effects of angiotensin-II on brain endothelial cell permeability via PPARalpha regulation of para- and trans-cellular pathways.
Application : WB
Reactivity : Human
Wang H et al. Am J Physiol Endocrinol Metab 2019; 316 (1):E54-E62 Adipose tissue transplantation ameliorates lipodystrophy-associated metabolic disorders in seipin-deficient mice.
Application : WB
Orkhon B et al. Mol Med Rep 2018; (Epub) Astragalus root induces ovarian β-oxidation and suppresses estrogen-dependent uterine proliferation.
Kubo H et al. Lipids Health Dis 2017; 16 (1):106 Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice.
Song L et al. Sci Rep 2017; 7:41604 Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet.
Chung IH et al. Oncotarget 2016; 7 (16):22448-59 ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance.
Application : WB
Reactivity : Human
Ye JH et al. Sci Rep 2016; 6:33102 Pentoxifylline ameliorates non-alcoholic fatty liver disease in hyperglycaemic and dyslipidaemic mice by upregulating fatty acid β-oxidation.
Application : WB
Xu P et al. Nutr Metab (Lond) 2015; 12:58 diet rich in docosahexaenoic acid/eicosapentaenoic acid robustly ameliorates hepatic steatosis and * resistance in seipin deficient lipodystrophy mice.
Application : WB
Hsu CY et al. Biogerontology 2014; Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees.
Application : WB
Reactivity : Queen Bee
Lai YS et al. J Agric Food Chem 2014; Garlic essential oil protects against obesity-triggered nonalcoholic fatty liver disease through modulation of lipid metabolism and oxidative stress.
Application : WB
Hsu CY et al. J Gerontol A Biol Sci Med Sci 2013; Changes in Energy-Regulated Molecules in the Trophocytes and Fat Cells of Young and Old Worker Honeybees (Apis mellifera).
Application : WB
Reactivity : Honeybee
Ho FM et al. J Ethnopharmacol 2012; 142 (1):175-87 Anti-atherosclerotic action of Ger-Gen-Chyn-Lian-Tang and AMPK-dependent lipid lowering effect in hepatocytes.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169