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PPAR delta antibody [N2C3]

Anti-PPAR delta antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113250
Anti-PPAR delta antibody [N2C3] used in Western Blot (WB). GTX113250
Anti-PPAR delta antibody [N2C3] used in Western Blot (WB). GTX113250

Cat No. GTX113250

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

K562 , HL-60 , DDDDK-tagged PPARD delta-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPAR delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037655

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peroxisome proliferator activated receptor delta , FAAR , NR1C2 , NUC1 , NUCI , NUCII , PPARB

Background

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPAR delta antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113250

GTX113250 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using PPAR delta(beta)(GTX113250) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPAR delta antibody [N2C3] used in Western Blot (WB). GTX113250

GTX113250 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR delta antibody [N2C3] (GTX113250) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PPAR delta antibody [N2C3] used in Western Blot (WB). GTX113250

GTX113250 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR delta antibody [N2C3] (GTX113250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

PPAR delta antibody [N2C3] Cat No. GTX113250

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 29215072
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Reprod Biol Endocrinol in 2018.PMID: 29316934
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf