Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 μg of whole cell lysate)
A: NT2D1
B: U87-MG
5% SDS PAGE
GTX114655 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PPP2R3A antibody [C2C3], C-term detects PPP2R3A protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPP2R3A(GTX114655) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min