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PPP2R5E antibody, C-term

Anti-PPP2R5E antibody, C-term used in Western Blot (WB). GTX89983

Cat No. GTX89983

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89983-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-LKRGLRRDGIIPT, from the C Terminus of the protein sequence according to NP_006237.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10724314

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 2 regulatory subunit B'epsilon , B56E , B56epsilon

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-PPP2R5E antibody, C-term used in Western Blot (WB). GTX89983

GTX89983 WB Image

WB analysis of human cerebellum (A), mouse (B) and rat (C) brain lysate using GTX89983 PPP2R5E antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf