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PSAT1 antibody [GT1353]

KO/KD
Anti-PSAT1 antibody [GT1353] used in Western Blot (WB). GTX633629
Anti-PSAT1 antibody [GT1353] used in IHC (Paraffin sections) (IHC-P). GTX633629
Anti-PSAT1 antibody [GT1353] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633629
Anti-PSAT1 antibody [GT1353] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633629
Anti-PSAT1 antibody [GT1353] used in Western Blot (WB). GTX633629
Anti-PSAT1 antibody [GT1353] used in Western Blot (WB). GTX633629

Cat. No. GTX633629

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1353

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PSAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

mouse brain , 293T

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSAT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888353

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoserine aminotransferase 1 , EPIP , NLS2 , PSA , PSAT , PSATD

Background

The protein encoded by this gene is likely a phosphoserine aminotransferase, based on similarity to proteins in mouse, rabbit, and Drosophila. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSAT1 antibody [GT1353] used in Western Blot (WB). GTX633629

GTX633629 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (GTX633629) diluted at 1:500.

Anti-PSAT1 antibody [GT1353] used in IHC (Paraffin sections) (IHC-P). GTX633629

GTX633629 IHC-P Image

PSAT1 antibody [GT1353] detects secreted PSAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PSAT1 stained by PSAT1 antibody [GT1353] (GTX633629) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PSAT1 antibody [GT1353] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633629

GTX633629 ICC/IF Image

PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSAT1 protein stained by PSAT1 antibody [GT1353] (GTX633629) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PSAT1 antibody [GT1353] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633629

GTX633629 ICC/IF Image

PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSAT1 stained by PSAT1 antibody [GT1353] (GTX633629) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PSAT1 antibody [GT1353] used in Western Blot (WB). GTX633629

GTX633629 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (GTX633629) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PSAT1 antibody [GT1353] used in Western Blot (WB). GTX633629

GTX633629 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (GTX633629) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169