ICC/IF analysis of human motor neurons derived from iPSCs using GTX22723 PSD95 antibody [6G6-1C9].
Fixation : 4% paraformaldehyde
Permeabilization : 0.2% Triton X-100 in PBS for 10 minutes
Dilution: 1:500 in 5% donkey serum overnight at 4ºC

ICC/IF analysis of U251 cells using GTX22723 PSD95 antibody [6G6-1C9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

FACS analysis of Neuro-2a cells using GTX22723 PSD95 antibody [6G6-1C9] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of cultured primary cortical neurons using GTX22723 PSD95 antibody [6G6-1C9].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% triton X-100 in HBSS for 10 minutes

ICC/IF analysis of C6 cells using GTX22723 PSD95 antibody [6G6-1C9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

FACS analysis of U87-MG cells using GTX22723 PSD95 antibody [6G6-1C9] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

WB analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates using GTX22723 PSD95 antibody [6G6-1C9].
Dilution : 1:200

ICC/IF analysis of HeLa cells using GTX22723 PSD95 antibody [6G6-1C9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

FACS analysis of SH-SY5Y cells using GTX22723 PSD95 antibody [6G6-1C9] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde