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PSMD10 antibody

Anti-PSMD10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48519
Anti-PSMD10 antibody used in Western Blot (WB). GTX48519
Anti-PSMD10 antibody used in Flow cytometry (FACS). GTX48519

Cat. No. GTX48519

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Pig
Package
50 μl ($399)
See all PSMD10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 1:500
FACS 1:500
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an internal portion of the human Gankyrin protein sequence (between residues 100-200). [UniProt# O75832]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10618114

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome 26S subunit, non-ATPase 10 , dJ889N15.2 , p28 , p28(GANK)

Cellular Localization

Cytoplasm,Nucleus

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSMD10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48519

GTX48519 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX48519 PSMD10 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:500

Anti-PSMD10 antibody used in Western Blot (WB). GTX48519

GTX48519 WB Image

WB analysis of HeLa cell lysate using GTX48519 PSMD10 antibody.

Anti-PSMD10 antibody used in Flow cytometry (FACS). GTX48519

GTX48519 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX48519 PSMD10 antibody.
Orange : Primary antibody
Blue : secondary antibody control
Dilution : 1:500

REFERENCE

Application Reference

Wang XH et al. Oncol Lett 2017; 13 (5) : 3239-3246 TGF-β1 signaling pathway serves a role in HepG2 cell regulation by affecting the protein expression of PCNA, gankyrin, p115, XIAP and survivin.
Wang X.H. et al. Oncol Lett 2017; 13 : 3239-3246 TGF-β1 signaling pathway serves a role in HepG2 cell regulation by affecting the protein expression of PCNA, gankyrin, p115, XIAP and survivin
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399