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PTBP1 antibody

Anti-PTBP1 antibody used in Immunoprecipitation (IP). GTX02176
Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176
Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176
Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176
Anti-PTBP1 antibody used in Western Blot (WB). GTX02176
Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176
Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176
Anti-PTBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02176
Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176

Cat No. GTX02176

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:300-1:10000
IHC-P 1:1000-1:5000
IP 1-4 μl/mg of lysate
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to the N-terminus of the Human PTBP1

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

polypyrimidine tract binding protein 1 , HNRNP-I , HNRNPI , HNRPI , PTB , PTB-1 , PTB-T , PTB2 , PTB3 , PTB4 , pPTB

Cellular Localization

Nucleus

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PTBP1 antibody used in Immunoprecipitation (IP). GTX02176

GTX02176 IP Image

IP analysis of Jurkat whole cell lysate using GTX02176 PTBP1 antibody.
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
50 % Protein G agarose : 15 μl

Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176

GTX02176 IHC-P Image

IHC-P analysis of mouse small intestine tissue section using GTX02176 PTBP1 antibody.
Dilution : 1:2000

Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176

GTX02176 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX02176 PTBP1 antibody.
Dilution : 1:2000

Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176

GTX02176 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX02176 PTBP1 antibody.
Dilution : 1:2000

Anti-PTBP1 antibody used in Western Blot (WB). GTX02176

GTX02176 WB Image

WB analysis of various samples using GTX02176 PTBP1 antibody.
Lane A : HepG2 whole cell lysate
Lane B : Jurkat whole cell lysate
Lane C : HeLa whole cell lysate
Lane D : A431 whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176

GTX02176 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX02176 PTBP1 antibody.
Dilution : 1:2000

Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176

GTX02176 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX02176 PTBP1 antibody.
Dilution : 1:2000

Anti-PTBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02176

GTX02176 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02176 PTBP1 antibody.
Red : Primary antibody
Dilution : 1:1000
Permeabilzation : 0.3% Triton X-100

Anti-PTBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02176

GTX02176 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX02176 PTBP1 antibody.
Dilution : 1:2000

REFERENCE

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REVIEW

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