Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Ser380) antibody (GTX133192) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Ser380) antibody (GTX133192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.