• Country / Location Selection

United States (US)

Plectin antibody

Anti-Plectin antibody used in Western Blot (WB). GTX130764
Anti-Plectin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130764

Cat. No. GTX130764










100 μl ($399),
25 μl ($169)


Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

532 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Mouse, Rat(>80% identity)





PBS, 1% BSA, 20% Glycerol


0.025% ProClin 300


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.


0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species



Recombinant protein encompassing a sequence within the C-terminus region of human Plectin. The exact sequence is proprietary.


Purified by antigen-affinity chromatography.






For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.



plectin , EBS1 , EBSMD , EBSND , EBSO , EBSOG , EBSPA , HD1 , LGMD2Q , LGMDR17 , PCN , PLEC1 , PLEC1b , PLTN

Cellular Localization

Cytoplasm , cytoskeleton


Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (for reviews see PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names have been superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 10780662, 20052759). It has been shown that the short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996, a number of groups reported that patients suffering from epidermolysis bullosa simplex with muscular dystrophy (EBS-MD) lacked plectin expression in skin and muscle tissues due to defects in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687, 8696340). Two other subtypes of plectin-related EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of plectin-related diseases see PMID: 15810881, 19945614. Mutations in the plectin gene related to human diseases should be named based on the position in NM_000445 (variant 1, isoform 1c), unless the mutation is located within one of the other alternative first exons, in which case the position in the respective Reference Sequence should be used.


Research Area


Anti-Plectin antibody used in Western Blot (WB). GTX130764

GTX130764 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Plectin antibody (GTX130764) diluted at 1:5000.

Anti-Plectin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130764

GTX130764 ICC/IF Image

Plectin antibody detects Plectin protein at cytoskeleton by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Plectin stained by Plectin antibody (GTX130764) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.


There are currently no references for Plectin antibody (GTX130764). Be the first to share your publications with this product.


Plectin antibody Cat. No. GTX130764

Rating ( Average 5 based on 2 users reviews)
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Jan-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U2OS cells
Blocking : 5% BSA, 5% goat serum and 0.01% Triton X-100 in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS, 25°C, 0.25Hr
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 25°C, 0.2Hr
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 05-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10 μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Package List Price ($)
$ 399
$ 169