GeneTex
United States (US)

Polycystin 2 (Active) recombinant protein

Cat No. GTX65204

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

259 nmol phosphate incorporated into CREBtide substrate peptide per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

~130.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥80%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PKD, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme2, PKD2, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2

Background

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain (1). It exhibits the strongest homology to the serine/threonine protein kinases PKD/PKCmu and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation (2).PKD2 can be activated by phorbol esters both in vivo and in vitro but also by gastrin via the cholecystokinin/CCK(B) receptor in human gastric cancer cells stably transfected with the CCK(B)/gastrin receptor (AGS-B cells) (3). Gastrin-stimulated PKD2 activation involves a heterotrimeric G alpha (q) protein as well as the activation of phospholipase C. Furthermore, PKD2 can be activated by classical and novel members of the protein kinase C (PKC) family such as PKC alpha , PKC epsilon, and PKC eta implicating PKD2 to be a downstream target of specific PKCs upon the stimulation of AGS-B cells with gastrin . PKD2 is predominantly cytoplasmic and stimulation of cells with the G protein-coupled receptor agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKD2 by a mechanism that requires PKC activity (4). In contrast to the other PKD isoenzymes, PKD2 activation does not induce its redistribution from the cytoplasm to the nucleus.

Research Area

Package List Price ($)
$ 349