GeneTex
United States (US)

Primary Antibody Diluent

Primary-Antibody-Diluent-GTX28208-IHC-1_18121411_341.jpg

Cat No. GTX28208

Reference 5
Application IHC
See all Antibody diluent products
PRODUCT

Product Description

Primary antibody diluent (with background reducing component)
APPLICATION

Application Note

Preparation of Reagent: Bring the diluent to room temperature and mix thoroughly before use. No further preparation is required. Procedure: 1) Pour the diluent in a clean glass container. 2) Add concentrated primary antibody directly in the diluent and mix thoroughly. 3) Diluted antibody is ready to use now and should be stored at 2-8¼ C for further use.
PROPERTIES

Form

Liquid

Storage

Keep as concentrated solution, aliquot and store at 4ºC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

In order to obtain an optimal staining, correct dilution as well as selection of appropriate diluent is very critical. Ionic strength, pH of the environment and the presence or absence of detergents can affect the binding as well as the stability of the primary antibody. The optimal pH together with stabilizing proteins in Primary Antibody Diluent ensures the maximum stability of the primary antibodies. Our Primary Antibody Diluent contains optimal concentrations of sodium chloride and detergents to prevent nonspecific binding and better penetration of primary antibodies respectively. Primary Antibody Diluent is specifically designed to dilute primary antibodies for immunohistochemistry. Both, mono and polyclonal antibodies can be diluted in this buffer. This product can be used as a diluent to prepare primary antibodies as well as negative controls. This diluent can be used with any primary antibody irrespective of its source of origin. Primary antibody diluent is specifically designed to stabilize the primary antibodies as well as to reduce the nonspecific background staining observed during the immunostaining.
DATA IMAGES
Primary-Antibody-Diluent-GTX28208-IHC-1_18121411_341.jpg

GTX28208 IHC Image


REFERENCE

Application Reference

Liu X et al. Ann Diagn Pathol 2018; 38 : 67-70 FOXP3 and CD25 double staining antibody cocktails identify regulatory T cells in different types of tumor tissues using tissue microarrays.
Hoogsteen IJ et al. Int J Radiat Oncol Biol Phys 2012; 84 (3) : 807-14 Expression of EGFR under tumor hypoxia: identification of a subpopulation of tumor cells responsible for aggressiveness and treatment resistance.
Nijkamp MM et al. Radiother Oncol 2011; 101 (1) : 165-70 Spatial relationship of phosphorylated epidermal growth factor receptor and activated AKT in head and neck squamous cell carcinoma.
Application : IHC
Hoeben BA et al. J Nucl Med 2010; 51 (7) : 1076-83 PET of hypoxia with 89Zr-labeled cG250-F(ab')2 in head and neck tumors.
Troost EG et al. J Nucl Med 2010; 51 (5) : 713-9 Histopathologic validation of 3'-deoxy-3'-18F-fluorothymidine PET in squamous cell carcinoma of the oral cavity.
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Package List Price ($)
$ 179