GeneTex
United States (US)

Prohibitin antibody

GTX101105_41808_20161201_WB_shRNA_watermark_19011506_878.jpg
GTX101105_42663_20161219_IHC-P_M_19011506_890.jpg
GTX101105_39820_WB_R_19011506_111.jpg
GTX101105_42663_20161219_IHC-P_R_19011506_816.jpg
GTX101105_WB_M_19011506_235.jpg
GTX101105_42781_20170419_IFA_19011506_530.jpg
GTX101105_IHC_19011506_234.jpg
GTX101105_39820_IFA_19011506_281.jpg
GTX101105_39820_20150327_IP_19011506_670.jpg
GTX101105_42663_20161110_WB_19011506_671.jpg
GTX101105_39820_20151223_IHC-P_19011506_655.jpg

Cat No. GTX101105

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , PC-12

Predict Reactivity

Zebrafish, Bovine, Cat, Dog, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prohibitin,Hel-215,Hel-S-54E,Phb1,Phb

Cellular Localization

Mitochondrion inner membrane

Background

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101105_41808_20161201_WB_shRNA_watermark_19011506_878.jpg

GTX101105 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101105_42663_20161219_IHC-P_M_19011506_890.jpg

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101105_39820_WB_R_19011506_111.jpg

GTX101105 WB Image

Prohibitin antibody detects PHB protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12% SDS-PAGE
Prohibitin antibody (GTX101105) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101105_42663_20161219_IHC-P_R_19011506_816.jpg

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101105_WB_M_19011506_235.jpg

GTX101105 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX101105 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101105_42781_20170419_IFA_19011506_530.jpg

GTX101105 ICC/IF Image

Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Prohibitin protein stained by Prohibitin antibody (GTX101105) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

GTX101105_IHC_19011506_234.jpg

GTX101105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Prohibitin(GTX101105) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101105_39820_IFA_19011506_281.jpg

GTX101105 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Prohibitin(GTX101105) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101105_39820_20150327_IP_19011506_670.jpg

GTX101105 IP Image

Immunoprecipitation of Prohibitin protein from 293T whole cell extracts using 5 μg of Prohibitin antibody (GTX101105).
Western blot analysis was performed using Prohibitin antibody (GTX101105).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101105_42663_20161110_WB_19011506_671.jpg

GTX101105 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:1000.

GTX101105_39820_20151223_IHC-P_19011506_655.jpg

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169