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Prohibitin antibody

KO/KD Orthogonal
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105
Anti-Prohibitin antibody used in Immunoprecipitation (IP). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

Cat. No. GTX101105

Reference 6
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Prohibitin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

NIH-3T3 , PC-12 , 293T

Predict Reactivity

Zebrafish, Bovine, Cat, Dog, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951209

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prohibitin , HEL-215 , HEL-S-54e , PHB1

Cellular Localization

Mitochondrion inner membrane

Background

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Prohibitin(GTX101105) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105

GTX101105 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Prohibitin(GTX101105) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Prohibitin antibody used in Immunoprecipitation (IP). GTX101105

GTX101105 IP Image

Immunoprecipitation of Prohibitin protein from 293T whole cell extracts using 5 μg of Prohibitin antibody (GTX101105).
Western blot analysis was performed using Prohibitin antibody (GTX101105).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

Prohibitin antibody detects PHB protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
Prohibitin antibody (GTX101105) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX101105 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105

GTX101105 ICC/IF Image

Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Prohibitin protein stained by Prohibitin antibody (GTX101105) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Prohibitin stained by Prohibitin antibody (GTX101105) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chiu YC et al. Biochem Biophys Rep 2021; 28 : 101166 Accumulation of cholesterol suppresses oxidative phosphorylation and altered responses to inflammatory stimuli of macrophages.
Application : ICC/IF
Reactivity : Mouse
Fang CH et al. J Biomed Sci 2020; 27 (1) : 42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.
Application : WB
Reactivity : Human
Heng-Hsiung Wu et al. Journal of Food and Drug Analysis 2019; (Epub) Involvement of prohibitin 1 and prohibitin 2 upregulation in cBSA-induced podocyte cytotoxicity.
Application : IHC-P
Reactivity : Mouse
Moriggi M et al. Proteomics 2017; (Epub) Contribution of Extracellular Matrix and Signal Mechanotransduction to Epithelial Cell Damage in Inflammatory Bowel Disease Patients: A Proteomic Study.
Application : WB
Reactivity : Human
Yurugi H et al. Oncogene 2017; (Epub) Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Prohibitin antibody Cat. No. GTX101105

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2020.PMID: 32169072
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2020.PMID: 32169072
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Kidney tissue
Fixation : Paraffin-embedded
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.5Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Mouse kidney, 16 week.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169