GeneTex
United States (US)

Punt antibody

Punt-antibody_WB_GTX14680-1_18121411_952.jpg

Cat No. GTX14680

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IP: Use at a dilution of >1/200. WB: Use at a dilution of >1/500. Detects a band of approximately 63 kDa Optimal dilutions/concentrations should be determined by the end user.

Positive Control

D. melanogaster extracts
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide.Supplied in antibody stabilization buffer.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide: QLLIKNHTNIDDAKESTNC, corresponding to C terminal amino acids 497-515 of Drosophila melanogaster Punt. Peptides were amidated for conjugation.

Purification

Immunogen affinity purified
Purified against immobilized antigen based affinity chromatography which yielded an epitope specific antibody.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Fruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog and wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promotes its cell division in the female germline. The Signaling by TGF beta related factors requires ligand induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the type I receptor is encoded by the thick veins (tkv) gene and the type II receptor is encoded by the punt (put) gene. These receptors mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta type factors. Over expression or mutation in dpp suppress germline stem cell differentiation. The Drosophila punt gene encodes a type II serine/threonine kinase TGF beta/Dpp (Decapentaplegic) receptor. Dpp actions are mediated by its receptor Punt and Saxophone. There are 5 down stream component in the dpp signaling cascade required to block the development of various organelles including salivary glands. Tthese are Mothers against dpp (Mad), Medea (Med) and Schnurri (Shn). Punt signaling is also responsible for calcium gradient formation during D. melanogaster development. Punt gene encodes for a homolog of vertebrate type II receptor and Punt, like thick veins (Tkv) is essential for in vivo dpp dependent patterning process. No Dpp dependent signal processing is apparent in the absence of Punt ot Tkv suggesting that both receptors acts in concert to transduce Dpp signaling.

Research Area

DATA IMAGES
Punt-antibody_WB_GTX14680-1_18121411_952.jpg

GTX14680 WB Image

Western blot of Punt on Punt-Flag constructs. Antibody dilutions 1:200 (GTX14679) and 1:500 (GTX14680). Arrow indicate migration of Punt-Flag
Lanes:
A & C. Punt serum (GTX14679)
B & D. GTX14679

Package List Price ($)
$ 319