Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with pygopus 2 antibody (GTX120215) diluted at 1:5000.

pygopus 2 antibody detects Pygo2 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung adenocarcinoma. pygopus 2 antibody (GTX120215) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX120215 diluted at 1:10000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with pygopus 2 antibody (GTX120215) diluted at 1:1000.