Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RABGAP1(GTX115742) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30 μg of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115742 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115742 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using RABGAP1(GTX115742) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min