WB analysis of HeLa whole cell lysate using GTX48751 RAD23B antibody.
Loading : 33 μg
Dilution : 1:500

Western blot using GeneTex's affinity purified anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate. Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown). Approximately 33 μg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4ºC with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.