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RAE1 antibody

Anti-RAE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33456
Anti-RAE1 antibody used in Western Blot (WB). GTX33456
Anti-RAE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33456
Anti-RAE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33456

Cat No. GTX33456

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-368 of human RAE1 (NP_003601.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribonucleic acid export 1 , MIG14 , MRNP41 , Mnrp41 , dJ481F12.3 , dJ800J21.1

Cellular Localization

Cytoplasm,Nucleus

Background

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33456

GTX33456 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX33456 RAE1 antibody.
Blue : DAPI

Anti-RAE1 antibody used in Western Blot (WB). GTX33456

GTX33456 WB Image

WB analysis of various sample lysates using GTX33456 RAE1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-RAE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33456

GTX33456 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33456 RAE1 antibody.
Dilution : 1:100

Anti-RAE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33456

GTX33456 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33456 RAE1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329