Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAMP2 antibody (GTX108524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SG xenograft, using RAMP2(GTX108524) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Mol Vis in 2012.PMID: 22690112