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RANKL antibody

Anti-RANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX32834
Anti-RANKL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32834
Anti-RANKL antibody used in Western Blot (WB). GTX32834

Cat No. GTX32834

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 78-317 of human TNFSF11 (NP_003692.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887682

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX32834

GTX32834 IHC-P Image

IHC-P analysis of human colon tissue using GTX32834 RANKL antibody.
Dilution : 1:100

Anti-RANKL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32834

GTX32834 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX32834 RANKL antibody.
Blue : DAPI
Dilution : 1:100

Anti-RANKL antibody used in Western Blot (WB). GTX32834

GTX32834 WB Image

WB analysis of various sample lysates using GTX32834 RANKL antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

REVIEW

RANKL antibody Cat No. GTX32834

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Arthritis Res Ther in 2016.PMID: 26785775
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339