GeneTex
United States (US)

RANKL antibody

GTX32834_20180530_WB_18121410_273.jpg
GTX32834_20180530_IHC-P_18121410_125.jpg

Cat No. GTX32834

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 78-317 of human TNFSF11 (NP_003692.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Superfamily Member 11,Cd254,Odf,Opgl,Optb2,Rankl,Tnlg6B,Trance,Hrankl2,Sodf,Tnfsf11

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced tra

Database

Research Area

DATA IMAGES
GTX32834_20180530_WB_18121410_273.jpg

GTX32834 WB Image

WB analysis of various samples using GTX32834 RANKL antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32834_20180530_IHC-P_18121410_125.jpg

GTX32834 IHC-P Image

IHC-P analysis of human colon tissue using GTX32834 RANKL antibody.
Dilution : 1:100

REFERENCE
Package List Price ($)
$ 329