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RANKL antibody

Anti-RANKL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32834
Anti-RANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX32834
Anti-RANKL antibody used in Western Blot (WB). GTX32834
Anti-RANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX32834

Cat. No. GTX32834

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RANKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 78-317 of human TNFSF11 (NP_003692.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887682

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RANKL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32834

GTX32834 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX32834 RANKL antibody.
Blue : DAPI
Dilution : 1:100

Anti-RANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX32834

GTX32834 IHC-P Image

IHC-P analysis of human colon tissue using GTX32834 RANKL antibody.
Dilution : 1:100

Anti-RANKL antibody used in Western Blot (WB). GTX32834

GTX32834 WB Image

WB analysis of various sample lysates using GTX32834 RANKL antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RANKL antibody used in IHC (Paraffin sections) (IHC-P). GTX32834

GTX32834 IHC-P Image

The data was published in the journal Arthritis Res Ther in 2016.PMID: 26785775

REFERENCE

Application Reference

X Zeng et al. Pharmacognosy Magazine 2023; Bee Pollen Stimulated BMP2 and RANK Signaling Pathway in Osteoprogenitors and Improved Bone Mineralization in Rat Model of Femur Fracture
BF Botelho et al. Photomed Laser Surg 2023; 41 (7) : 328-342 Biomodulation of Induced Tooth Movement by Three Methods, Corticopuncture, Photobiomodulation, and Their Combination: An Animal Study
Application : IHC-P
Reactivity : Rat
Jiah Yeom et al. Microorganisms 2021; 9 (4) Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio.
Application : WB
Reactivity : Human
Mei-Chi Chang et al. pharmacology & toxicology 2018; (Epub) Butyrate Stimulates Histone H3 Acetylation, RANKL Expression, But Inhibited Osteoprotegerin Expression/Secretion in MG-63 Osteoblastic Cells.
Application : WB
Reactivity : Human
Beckmann J et al. Arthritis Res Ther 2016; 18 : 17 Effect of M3 muscarinic acetylcholine receptor deficiency on collagen antibody-induced arthritis.
Application : IHC-P
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399