Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12V Mutant) antibody [HL169] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RAS (G12V Mutant) antibody [HL169] detects RAS (G12V Mutant) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BxPC-3 xenograft (left) and CFPAC-1 xenograft (right).
RAS (G12V Mutant) stained by RAS (G12V Mutant) antibody [HL169] (GTX635623) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min