A431 cells were labeled with anti-RASSF1A (clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj streptavidin.

Cell lysates of HEk293(1), A549(2) and HeLa(3) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection