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RBMS3 antibody

Anti-RBMS3 antibody used in Western Blot (WB). GTX00828
Anti-RBMS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00828
Anti-RBMS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00828
Anti-RBMS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00828
Anti-RBMS3 antibody used in Western Blot (WB). GTX00828
Anti-RBMS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00828

Cat No. GTX00828

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
ELISA 0.1-0.5μg/ml

Note :

IHC-P
Recommend using heat mediated antigen retrieval.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human RBMS3 recombinant protein (Position: T350-P437).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RBMS3 , RNA binding motif single stranded interacting protein 3

Cellular Localization

Cytoplasm

Background

This gene encodes an RNA-binding protein that belongs to the c-myc gene single-strand binding protein family. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The encoded protein was isolated by virtue of its binding to an upstream element of the alpha2(I) collagen promoter. The observation that this protein localizes mostly in the cytoplasm suggests that it may be involved in a cytoplasmic function such as controlling RNA metabolism, rather than transcription. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-RBMS3 antibody used in Western Blot (WB). GTX00828

GTX00828 WB Image

WB analysis of various sample lysates using GTX00828 RBMS3 antibody.
Lane 1 : U2OS whole cell lysates
Lane 2 : PC-3 whole cell lysates
Lane 3 : U-87MG whole cell lysates
Lane 4 : A549 whole cell lysates
Lane 5 : HeLa whole cell lysates
Dilution : 0.5μg/mL
Loading : 50μg of sample under reducing condition

Anti-RBMS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00828

GTX00828 IHC-P Image

IHC-P analysis of human oesophagus squama cancer tissue using GTX00828 RBMS3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/mL

Anti-RBMS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00828

GTX00828 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX00828 RBMS3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/mL

Anti-RBMS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX00828

GTX00828 IHC-P Image

IHC-P analysis of rat brain tissue using GTX00828 RBMS3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/mL

Anti-RBMS3 antibody used in Western Blot (WB). GTX00828

GTX00828 WB Image

WB analysis of various sample lysates using GTX00828 RBMS3 antibody.
Lane 1 : rat heart tissue lysates
Lane 2 : mouse testicular tissue lysates
Dilution : 0.5μg/mL
Loading : 50μg of sample under reducing condition

Anti-RBMS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00828

GTX00828 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00828 RBMS3 antibody. Enzyme antigen retrieval was performed for 15 mins.
Red : Primary antibody
Blue : DAPI
Dilution : 2μg/mL

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 329