United States (US)

RIP2 (Active) recombinant protein

Cat No. GTX65297

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human

Application Note

20 nmol phosphate incorporated into MBP per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

59.0 kDa. ( Note )




50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol


Store at -80ºC. Product is stable for at least 6-12 months.


0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species


Expression System

Baculovirus (Sf9 insect cells)


Purity was assessed by SDS-PAGE (≥90%) and by HPLC.


For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


RIPK, receptorinteracting serinethreonine kinase2, receptor-interacting serine-threonine kinase 2, RIPK2, receptorinteracting serinethreonine kinase 2


RIPK2 (RIP2; RICK) is a death domain-containing protein kinase. Inohara identified cDNAs encoding a predicted 540-amino acid protein RICK, which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. Inohara also demonstrated that RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway (1). McCarthy found that overexpression of RIP2 signaled both cell death and NF-kappa-B activation (2). Thome reported that RICK specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (3). Chin generated Ripk2-deficient mice and concluded that RIPK2 is implicated in the innate response to pathogens by NOD and TLR-induced cell signaling and mediates cytokine-induced Ifng production in Th1 and NK cells (4). Also Kobayashi demonstrated that RIPK2 is required for signaling through both TLR and NOD protein family members in the innate immune system as well as for appropriate TCR signaling in the adaptive immune response (5).

Research Area

Package List Price ($)
$ 349