Immunoprecipitation of RNF40 protein from HeLa whole cell extracts using 5 μg of RNF40 antibody [N1N2], N-term (GTX115295).
Western blot analysis was performed using RNF40 antibody [N1N2], N-term (GTX115295).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RNF40(GTX115295) antibody at 1:500 dilution.

RNF40 antibody detects RNF40 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNF40 antibody (GTX115295) diluted by 1:1000.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RNF40(GTX115295) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115295 diluted at 1:1000