Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [HL2559] (GTX638936) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [HL2559] (GTX638936) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

ROBO1 antibody [HL2559] detects ROBO1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ROBO1 stained by ROBO1 antibody [HL2559] (GTX638936) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ROBO1 antibody [HL2559] detects ROBO1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
ROBO1 stained by ROBO1 antibody [HL2559] (GTX638936) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min