ROBO1 antibody

Cat No. GTX114103

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Chicken
Package	100 μl ($329), 25 μl ($169)

See all ROBO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , MDA-Mb-361 , HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ROBO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166472

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

roundabout guidance receptor 1 , DUTT1 , SAX3

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 6091 ROBO1
UniProt: Q9Y6N7 ROBO1

Research Area

DATA IMAGES

GTX114103 WB Image

HeLa whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000.

GTX114103 WB Image

Sample (15 μg of whole cell lysate)
A: MDA-Mb-361
B: HCC1937 Lx ( Lentivirus infection, carry contol vector)
C: HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)
GTX114103 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114103 IHC-P Image

ROBO1 antibody detects ROBO1 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ROBO1 antibody (GTX114103) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114103 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX114103 ICC/IF Image

ROBO1 antibody detects ROBO1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROBO1 stained by ROBO1 antibody (GTX114103) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.