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GeneTex
United States (US)

ROBO1 antibody

Orthogonal
GTX114103_42508_20160714_WB_Fraction_19011519_335.jpg
GTX114103_40968_CT_WB_18121410_965.jpg
GTX114103_42494_20160519_WB_18121410_182.jpg
GTX114103_40968_20141009_IHC_M_18121410_355.jpg

Cat No. GTX114103

Reference 3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Chicken
See all ROBO1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , MDA-Mb-361 , HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ROBO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Roundabout Guidance Receptor 1,Dutt1,Sax3,Robo1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114103_42508_20160714_WB_Fraction_19011519_335.jpg

GTX114103 WB Image

HeLa whole cell and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000.
GTX114103_40968_CT_WB_18121410_965.jpg

GTX114103 WB Image

Sample (15 µg of whole cell lysate)
A: MDA-Mb-361
B: HCC1937 Lx ( Lentivirus infection, carry contol vector)
C: HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)
GTX114103 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX114103_42494_20160519_WB_18121410_182.jpg

GTX114103 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000.
GTX114103_40968_20141009_IHC_M_18121410_355.jpg

GTX114103 IHC-P Image

ROBO1 antibody detects ROBO1 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ROBO1 antibody (GTX114103) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Yang T et al. Cell Rep 2018; 24 (10) : 2694-2708.e6 miR-92 Suppresses Robo1 Translation to Modulate Slit Sensitivity in Commissural Axon Guidance.
Application : ICC/IF
Reactivity : Chicken
Perez-Ortiz A et al. Oncotarget 2017; (Epub ahead of print) Pharmacogenetics of response to neoadjuvant paclitaxel treatment for locally advanced breast cancer.
Application : WB
Reactivity : Human
Choi YJ et al. Pathol Oncol Res 2014; 20 (3) : 517-9 Down-regulation of ROBO2 expression in prostate cancers.
Application : IHC-P
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169