APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , MDA-Mb-361 , HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)
Predict Reactivity
Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ROBO1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Roundabout Guidance Receptor 1,Dutt1,Sax3,Robo1
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
DATA IMAGES
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GTX114103 WB Image
HeLa whole cell and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000.
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GTX114103 WB Image
Sample (15 µg of whole cell lysate)
A: MDA-Mb-361
B: HCC1937 Lx ( Lentivirus infection, carry contol vector)
C: HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1)
GTX114103 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114103 WB Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody (GTX114103) diluted at 1:1000.
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GTX114103 IHC-P Image
ROBO1 antibody detects ROBO1 protein at cytosol on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ROBO1 antibody (GTX114103) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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