APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse liver , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Sheep, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951746
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
replication protein A1 , HSSB , MST075 , REPA1 , RF-A , RP-A , RPA70
Cellular Localization
Nucleus
Background
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Database
Research Area
DATA IMAGES
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GTX108749 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (GTX108749) antibody at 1:200 dilution.
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GTX108749 WB Image
RPA70 antibody detects RPA70 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody (GTX108749) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108749 IHC-P Image
RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung papillary adenocarcinoma. RPA70 antibody [C1C3] (GTX108749) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX108749 IP Image
Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3] (GTX108749). Western blot analysis was performed using RPA70 antibody [C1C3] (GTX108749). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX108749 IHC-P Image
RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPA70 antibody [C1C3] (GTX108749) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX108749 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (GTX108749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108749 WB Image
Sample (50 μg of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX108749 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108749 WB Image
RPA70 antibody [C1C3] detects RPA70 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody [C1C3] (GTX108749) diluted at 1:1000.
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REFERENCE
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