RPA70 antibody [C1C3]

Name: RPA70 antibody [C1C3]
Brand: GeneTex
SKU: GTX108749
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-RPA70 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108749

Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749

Anti-RPA70 antibody [C1C3] used in Immunoprecipitation (IP). GTX108749

Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

Cat. No. GTX108749

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all RPA70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951746

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication protein A1 , HSSB , MST075 , REPA1 , RF-A , RP-A , RPA70

Cellular Localization

Nucleus

Background

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Database

Gene ID: 6117 RPA1
UniProt: P27694 RPA1

Research Area

DATA IMAGES

GTX108749 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (GTX108749) antibody at 1:200 dilution.

GTX108749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (GTX108749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108749 IP Image

Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3] (GTX108749).
Western blot analysis was performed using RPA70 antibody [C1C3] (GTX108749).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108749 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108749 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108749 WB Image

RPA70 antibody [C1C3] detects RPA70 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody [C1C3] (GTX108749) diluted at 1:1000.

REFERENCE

Application Reference

Chen WT et al. J Biomed Sci 2021; 28 (1) : 81 Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation.

Application : ICC/IF

Reactivity : Mouse

Pietro Pichierri et al. bioRxiv 2018; (Epub) RAD51 AND MITOTIC FUNCTION OF MUS81 ARE ESSENTIAL FOR RECOVERY FROM LOW-DOSE OF CAMPTOTHECIN IN THE ABSENCE OF THE WRN EXONUCLEASE.

Application : WB

Reactivity : Human

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REVIEW