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RPA70 antibody [C1C3]

Anti-RPA70 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108749
Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749
Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749
Anti-RPA70 antibody [C1C3] used in Immunoprecipitation (IP). GTX108749
Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749
Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749
Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749
Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

Cat. No. GTX108749

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RPA70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951746

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

replication protein A1 , HSSB , MST075 , REPA1 , RF-A , RP-A , RPA70

Cellular Localization

Nucleus

Background

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Database

Research Area

DATA IMAGES

Anti-RPA70 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108749

GTX108749 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (GTX108749) antibody at 1:200 dilution.

Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

GTX108749 WB Image

RPA70 antibody detects RPA70 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody (GTX108749) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPA70 antibody [C1C3] used in Immunoprecipitation (IP). GTX108749

GTX108749 IP Image

Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3] (GTX108749).
Western blot analysis was performed using RPA70 antibody [C1C3] (GTX108749).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749

GTX108749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (GTX108749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

GTX108749 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108749 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

GTX108749 WB Image

RPA70 antibody [C1C3] detects RPA70 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody [C1C3] (GTX108749) diluted at 1:1000.

REFERENCE

Application Reference

Chen WT et al. J Biomed Sci 2021; 28 (1) : 81 Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation.
Application : ICC/IF
Reactivity : Mouse
Pietro Pichierri et al. bioRxiv 2018; (Epub) RAD51 AND MITOTIC FUNCTION OF MUS81 ARE ESSENTIAL FOR RECOVERY FROM LOW-DOSE OF CAMPTOTHECIN IN THE ABSENCE OF THE WRN EXONUCLEASE.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169