GeneTex
United States (US)

RPA70 antibody [C1C3]

GTX108749_IFA_19011507_373.jpg
GTX108749_41871_20141128_WB_19011507_112.jpg
GTX108749_41871_20151227_IHC-P_19011507_530.jpg
GTX108749_41871_20150327_IP_19011507_782.jpg
GTX108749_42242_20151102_IHC-P_M_19011507_666.jpg
GTX108749_IHC_19011507_570.jpg
GTX108749_39988_WB_M_liver_19011507_393.jpg

Cat No. GTX108749

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse liver

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Concentration

0.4mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HSSB antibody, MST075 antibody, REPA1 antibody, RF-A antibody, RP-A antibody, RPA70 antibody, RPA1 antibody, single-stranded DNA-binding protein antibody, replication protein A 70 kDa DNA-binding subunit antibody, replication factor A protein 1 antibody, RP-A p70 antibody, RF-A protein 1 antibody, MSTP075 antibody, "replication protein A1, 70kDa antibody"

Cellular Localization

Nucleus

Background

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
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Database

Research Area

DATA IMAGES
GTX108749_IFA_19011507_373.jpg

GTX108749 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (GTX108749) antibody at 1:200 dilution.

GTX108749_41871_20141128_WB_19011507_112.jpg

GTX108749 WB Image

RPA70 antibody detects RPA70 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody (GTX108749) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108749_41871_20151227_IHC-P_19011507_530.jpg

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108749_41871_20150327_IP_19011507_782.jpg

GTX108749 IP Image

Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3] (GTX108749).
Western blot analysis was performed using RPA70 antibody [C1C3] (GTX108749).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108749_42242_20151102_IHC-P_M_19011507_666.jpg

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108749_IHC_19011507_570.jpg

GTX108749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (GTX108749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108749_39988_WB_M_liver_19011507_393.jpg

GTX108749 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108749 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf