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RPE65 antibody [N1C3]

Anti-RPE65 antibody [N1C3] used in Western Blot (WB). GTX103472
Anti-RPE65 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103472

Cat No. GTX103472

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all RPE65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Rat, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPE65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037911

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

retinoid isomerohydrolase RPE65 , BCO3 , LCA2 , RP20 , mRPE65 , p63 , rd12 , sRPE65

Cellular Localization

Cytoplasm , Cell membrane; Lipid-anchor

Background

This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPE65 antibody [N1C3] used in Western Blot (WB). GTX103472

GTX103472 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RPE65 antibody [N1C3] (GTX103472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPE65 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103472

GTX103472 IHC-P Image

RPE65 antibody [N1C3] detects RPE65 protein at retinal pigment epithelium by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: RPE65 stained by RPE65 antibody [N1C3] (GTX103472) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuronal marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Nicholas J Esposito et al. Invest Ophthalmol Vis Sci 2021; 62 (2) : 7 Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3.
Yu C et al. J Cell Sci 2019; 132 (20) Annexin A5 regulates surface 帢v帣5 integrin for retinal clearance phagocytosis.
Muller C et al. Stem Cell Reports 2018; (Epub) Quantified F-Actin Morphology Is Predictive of Phagocytic Capacity of Stem Cell-Derived Retinal Pigment Epithelium.
Application : ICC/IF
Reactivity : Human
Bulloj A et al. Mol Neurobiol 2017; Semaphorin4D-PlexinB1 Signaling Attenuates Photoreceptor Outer Segment Phagocytosis by Reducing Rac1 Activity of RPE Cells.
Application : WB
Reactivity : Mouse
Yu CC et al. Free Radic Biol Med 2012; 52 (3) : 660-70 Dietary antioxidants prevent age-related retinal pigment epithelium actin damage and blindness in mice lacking αvβ5 integrin.
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169