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RPE65 antibody

Anti-RPE65 antibody used in IHC (Paraffin sections) (IHC-P). GTX64456
Anti-RPE65 antibody used in IHC (Paraffin sections) (IHC-P). GTX64456
Anti-RPE65 antibody used in Western Blot (WB). GTX64456

Cat. No. GTX64456

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RPE65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 274-533 of human RPE65 (NP_000320.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

retinoid isomerohydrolase RPE65 , BCO3 , LCA2 , RP20 , mRPE65 , p63 , rd12 , sRPE65

Cellular Localization

Cytoplasm,Cell membrane

Background

The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017]

Database

Research Area

DATA IMAGES

Anti-RPE65 antibody used in IHC (Paraffin sections) (IHC-P). GTX64456

GTX64456 IHC-P Image

IHC-P analysis of mouse eye tissue using GTX64456 RPE65 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RPE65 antibody used in IHC (Paraffin sections) (IHC-P). GTX64456

GTX64456 IHC-P Image

IHC-P analysis of rat eye tissue using GTX64456 RPE65 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RPE65 antibody used in Western Blot (WB). GTX64456

GTX64456 WB Image

WB analysis of various sample lysates using GTX64456 RPE65 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399