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RPL4 antibody

Anti-RPL4 antibody used in Western Blot (WB). GTX112184
Anti-RPL4 antibody used in Western Blot (WB). GTX112184
Anti-RPL4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112184
Anti-RPL4 antibody used in IHC (Paraffin sections) (IHC-P). GTX112184
Anti-RPL4 antibody used in IHC (Paraffin sections) (IHC-P). GTX112184
Anti-RPL4 antibody used in IHC (Paraffin sections) (IHC-P). GTX112184

Cat. No. GTX112184

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RPL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Raji , K562 , NCI-H929

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167509

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L4 , L4

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL4 antibody used in Western Blot (WB). GTX112184

GTX112184 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RPL4 antibody (GTX112184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPL4 antibody used in Western Blot (WB). GTX112184

GTX112184 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
B: K562
C: NCI-H929
10% SDS PAGE
GTX112184 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPL4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112184

GTX112184 ICC/IF Image

RPL4 antibody detects RPL4 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: RPL4 protein stained by RPL4 antibody (GTX112184) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-RPL4 antibody used in IHC (Paraffin sections) (IHC-P). GTX112184

GTX112184 IHC-P Image

RPL4 antibody detects RPL4 protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
RPL4 antibody (GTX112184) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL4 antibody used in IHC (Paraffin sections) (IHC-P). GTX112184

GTX112184 IHC-P Image

RPL4 antibody detects RPL4 protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RPL4 antibody (GTX112184) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL4 antibody used in IHC (Paraffin sections) (IHC-P). GTX112184

GTX112184 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RPL4(GTX112184) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169