RUNX1 antibody [HL2211] detects RUNX1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

RUNX1 antibody [HL2211] detects RUNX1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

RUNX1 antibody [HL2211] detects RUNX1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.