Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 μg of RUVBL1 antibody (GTX111294).
Western blot analysis was performed using RUVBL1 antibody (GTX111294).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

RUVBL1 antibody detects RUVBL1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RUVBL1 antibody detects RUVBL1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RUVBL1(GTX111294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min