APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5μg/mL - 2μg/mL |
0.5μg/mL - 2μg/mL |
Assay dependent |
50ng/well - 200ng/well (for Capture) ; 0.05μg/mL - 0.2μg/mL (for Detection) |
Assay dependent |
Note :
Sandwich ELISA
Capture : GTX60866, Detection : GTX60920
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Not tested in other applications.
Product Note
This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 50% Glycerol
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human IgM
Target Isotype
IgM
Purification
Protein A purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Background
Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Database
Research Area
DATA IMAGES
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GTX60866 ELISA Image
Sandwich ELISA analysis of human IgM using GTX60866 Human IgM antibody [RM121] as the capture antibody (100ng/well) and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.
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GTX60866 ELISA Image
Sandwich ELISA analysis of human plasma using GTX60866 Human IgM antibody [RM121] as the capture antibody (100ng/well) and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.
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GTX60866 ELISA Image
ELISA analysis of human immunoglobulins (50 ng/well) using serial diluted GTX60866 Human IgM antibody [RM121]. No cross reactivity with Human IgG, IgE, IgD, or IgA. Dilution : 200 ng/mL, 50 ng/mL of RM121 was used as the primary antibody.
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GTX60866 ELISA Image
ELISA analysis of different amount of immunogen proteins using serial diluted GTX60866 Human IgM antibody [RM121].
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REFERENCE
There are currently no references for Rabbit Anti-Human IgM antibody [RM121] (GTX60866). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rabbit Anti-Human IgM antibody [RM121] (GTX60866). Be the first to share your experience with this product.