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Rabbit Anti-Human IgM antibody [RM121]

Rabbit Anti-Human IgM antibody [RM121]. GTX60866
Rabbit Anti-Human IgM antibody [RM121]. GTX60866
Rabbit Anti-Human IgM antibody [RM121]. GTX60866
Rabbit Anti-Human IgM antibody [RM121]. GTX60866

Cat No. GTX60866

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM121

Isotype

IgG

Application

ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5μg/mL - 2μg/mL
IHC-P 0.5μg/mL - 2μg/mL
FACS Assay dependent
ELISA 50ng/well - 200ng/well (for Capture) ; 0.05μg/mL - 0.2μg/mL (for Detection)
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX60866, Detection : GTX60920

Not tested in other applications.

Product Note

This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human IgM

Target Isotype

IgM

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]

Database

  • Gene ID: 3507 IGHM

Research Area

DATA IMAGES

Rabbit Anti-Human IgM antibody [RM121]. GTX60866

GTX60866 ELISA Image

ELISA analysis of human immunoglobulins (50 ng/well) using serial diluted GTX60866 Human IgM antibody [RM121]. No cross reactivity with Human IgG, IgE, IgD, or IgA.
Dilution : 200 ng/mL, 50 ng/mL of RM121 was used as the primary antibody.

Rabbit Anti-Human IgM antibody [RM121]. GTX60866

GTX60866 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX60866 Human IgM antibody [RM121].

Rabbit Anti-Human IgM antibody [RM121]. GTX60866

GTX60866 ELISA Image

Sandwich ELISA analysis of human IgM using GTX60866 Human IgM antibody [RM121] as the capture antibody (100ng/well) and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

Rabbit Anti-Human IgM antibody [RM121]. GTX60866

GTX60866 ELISA Image

Sandwich ELISA analysis of human plasma using GTX60866 Human IgM antibody [RM121] as the capture antibody (100ng/well) and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.

REFERENCE

There are currently no references for Rabbit Anti-Human IgM antibody [RM121] (GTX60866). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 379