GeneTex
United States (US)

Rad17 antibody

Anti-Rad17 antibody used in Western Blot (WB). GTX33453
Anti-Rad17 antibody used in Immunoprecipitation (IP). GTX33453
Anti-Rad17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33453

Cat No. GTX33453

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rad17 Checkpoint Clamp Loader Component,Ccyc,Hrad17,R24L,Rad17Sp,Rad24,Rad17

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul

Database

Research Area

DATA IMAGES
Anti-Rad17 antibody used in Western Blot (WB). GTX33453

GTX33453 WB Image

WB analysis of various samples using GTX33453 Rad17 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Rad17 antibody used in Immunoprecipitation (IP). GTX33453

GTX33453 IP Image

IP analysis of K562 cell lysate using GTX33453 Rad17 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Anti-Rad17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33453

GTX33453 ICC/IF Image

ICC/IF analysis of A549 cell using GTX33453 Rad17 antibody.

Package List Price ($)
$ 329