HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of Rad50 protein from NT2D1 whole cell extract using 5 μg of Rad50 antibody [N1N2], N-term (GTX119731).
Western blot analysis was performed using Rad50 antibody [N1N2], N-term (GTX119731).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rad50(GTX119731) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rad50 antibody [N1N2], N-term detects RAD50 protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg GL261 whole cell lysate/extract
5% SDS-PAGE
Rad50 antibody [N1N2], N-term (GTX119731) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: NT2D1
5% SDS PAGE
GTX119731 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.