Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad51 protein stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Rad51 antibody [N1C2] detects Rad51 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Rad51 stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.

Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad51 stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rad51 antibody [N1C2] immunoprecipitates Rad51 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rad51 protein by 4 μg Rad51 antibody [N1C2] (GTX100469)
5 % SDS-PAGE
The immunoprecipitated Rad51 protein was detected by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal PLoS One in 2013. PMID: 23724141

The data was published in the journal Nat Commun in 2019. PMID: 30874560

The data was published in the journal PLoS One in 2015. PMID: 26683461

The data was published in the journal Nat Commun in 2019. PMID: 30874560