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Rat RPP40 protein (active)

Rat RPP40 protein (active). GTX00344-pro
Rat RPP40 protein (active). GTX00344-pro
Rat RPP40 protein (active). GTX00344-pro

Cat No. GTX00344-pro

Application Functional Assay
Species Rat
Package
10 μg ($299)
APPLICATION

Application Note

Ribonuclease P is a site specific endonuclease that generates mature tRNAs by catalysing the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. In bacteria, RNase P consists of of two components: a large RNA (about 400 base pairs) encoded by rnpB, and a small protein (119 to 133 amino acids) encoded by rnpA. The RNA moiety of RNase P carries the catalytic activity; the protein component plays an auxiliary, but essential, role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. The sequence of rnpA is not highly conserved, however there is, in the central part of the protein, a conserved basic region. Besides, Nucleophosmin (NPM) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat RNASEP and recombinant rat NPM. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NPM-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of RNASEP and NPM was in a dose dependent manner.

Observed MW

23 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Met91~Asp259 (NP_001013073.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ribonuclease P/MRP subunit p40

Database

DATA IMAGES
Rat RPP40 protein (active). GTX00344-pro

GTX00344-pro Functional Assay Image

Functional ELISA analysis of GTX00344-pro Rat RPP40 protein (active) which can bind immobilized NPM protein.

Rat RPP40 protein (active). GTX00344-pro

GTX00344-pro Image

SDS-PAGE analysis of GTX00344-pro Rat RPP40 protein (active).

Rat RPP40 protein (active). GTX00344-pro

GTX00344-pro Image

WB analysis of GTX00344-pro Rat RPP40 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 299