Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RbAp48 + RbAp46(GTX70236) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RbAp48 + RbAp46(GTX70236) antibody at 1:200 dilution.

RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RbAp48 + RbAp46 antibody [15G12] detects RbAp48 + RbAp46 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody [15G12] (GTX70236) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Scale bar= 10μm.